Channel-Inactivating Mutations and Their Revertant Mutants in the Envelope Protein of Infectious Bronchitis Virus
Identifieur interne : 000F24 ( Main/Exploration ); précédent : 000F23; suivant : 000F25Channel-Inactivating Mutations and Their Revertant Mutants in the Envelope Protein of Infectious Bronchitis Virus
Auteurs : Janet To [Singapour] ; Wahyu Surya [Singapour] ; To Sing Fung [Singapour] ; Yan Li [Singapour] ; Carmina Verdià-Bàguena [Espagne] ; Maria Queralt-Martin [Espagne] ; Vicente M. Aguilella [Espagne] ; Ding Xiang Liu [Singapour] ; Jaume Torres [Singapour]Source :
- Journal of Virology [ 0022-538X ] ; 2017.
Abstract
It has been shown previously in the severe acute respiratory syndrome coronavirus (SARS-CoV) that two point mutations, N15A and V25F, in the transmembrane domain (TMD) of the envelope (E) protein abolished channel activity and led to
Url:
DOI: 10.1128/JVI.02158-16
PubMed: 27974570
PubMed Central: 5309962
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000717
- to stream Pmc, to step Curation: 000717
- to stream Pmc, to step Checkpoint: 000428
- to stream Ncbi, to step Merge: 000666
- to stream Ncbi, to step Curation: 000666
- to stream Ncbi, to step Checkpoint: 000666
- to stream Main, to step Merge: 000F26
- to stream Main, to step Curation: 000F24
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Channel-Inactivating Mutations and Their Revertant Mutants in the Envelope Protein of Infectious Bronchitis Virus</title>
<author><name sortKey="To, Janet" sort="To, Janet" uniqKey="To J" first="Janet" last="To">Janet To</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">School of Biological Sciences, Nanyang Technological University, Singapore, Singapore</nlm:aff>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>School of Biological Sciences, Nanyang Technological University, Singapore</wicri:regionArea>
<wicri:noRegion>Singapore</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Surya, Wahyu" sort="Surya, Wahyu" uniqKey="Surya W" first="Wahyu" last="Surya">Wahyu Surya</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">School of Biological Sciences, Nanyang Technological University, Singapore, Singapore</nlm:aff>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>School of Biological Sciences, Nanyang Technological University, Singapore</wicri:regionArea>
<wicri:noRegion>Singapore</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Fung, To Sing" sort="Fung, To Sing" uniqKey="Fung T" first="To Sing" last="Fung">To Sing Fung</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">School of Biological Sciences, Nanyang Technological University, Singapore, Singapore</nlm:aff>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>School of Biological Sciences, Nanyang Technological University, Singapore</wicri:regionArea>
<wicri:noRegion>Singapore</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Li, Yan" sort="Li, Yan" uniqKey="Li Y" first="Yan" last="Li">Yan Li</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">School of Biological Sciences, Nanyang Technological University, Singapore, Singapore</nlm:aff>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>School of Biological Sciences, Nanyang Technological University, Singapore</wicri:regionArea>
<wicri:noRegion>Singapore</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Verdia Baguena, Carmina" sort="Verdia Baguena, Carmina" uniqKey="Verdia Baguena C" first="Carmina" last="Verdià-Bàguena">Carmina Verdià-Bàguena</name>
<affiliation wicri:level="1"><nlm:aff id="aff2">Department of Physics, Laboratory of Molecular Biophysics. Universitat Jaume I, Castelló, Spain</nlm:aff>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Department of Physics, Laboratory of Molecular Biophysics. Universitat Jaume I, Castelló</wicri:regionArea>
<wicri:noRegion>Castelló</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Queralt Martin, Maria" sort="Queralt Martin, Maria" uniqKey="Queralt Martin M" first="Maria" last="Queralt-Martin">Maria Queralt-Martin</name>
<affiliation wicri:level="1"><nlm:aff id="aff2">Department of Physics, Laboratory of Molecular Biophysics. Universitat Jaume I, Castelló, Spain</nlm:aff>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Department of Physics, Laboratory of Molecular Biophysics. Universitat Jaume I, Castelló</wicri:regionArea>
<wicri:noRegion>Castelló</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Aguilella, Vicente M" sort="Aguilella, Vicente M" uniqKey="Aguilella V" first="Vicente M." last="Aguilella">Vicente M. Aguilella</name>
<affiliation wicri:level="1"><nlm:aff id="aff2">Department of Physics, Laboratory of Molecular Biophysics. Universitat Jaume I, Castelló, Spain</nlm:aff>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Department of Physics, Laboratory of Molecular Biophysics. Universitat Jaume I, Castelló</wicri:regionArea>
<wicri:noRegion>Castelló</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Liu, Ding Xiang" sort="Liu, Ding Xiang" uniqKey="Liu D" first="Ding Xiang" last="Liu">Ding Xiang Liu</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">School of Biological Sciences, Nanyang Technological University, Singapore, Singapore</nlm:aff>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>School of Biological Sciences, Nanyang Technological University, Singapore</wicri:regionArea>
<wicri:noRegion>Singapore</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Torres, Jaume" sort="Torres, Jaume" uniqKey="Torres J" first="Jaume" last="Torres">Jaume Torres</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">School of Biological Sciences, Nanyang Technological University, Singapore, Singapore</nlm:aff>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>School of Biological Sciences, Nanyang Technological University, Singapore</wicri:regionArea>
<wicri:noRegion>Singapore</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">27974570</idno>
<idno type="pmc">5309962</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5309962</idno>
<idno type="RBID">PMC:5309962</idno>
<idno type="doi">10.1128/JVI.02158-16</idno>
<date when="2017">2017</date>
<idno type="wicri:Area/Pmc/Corpus">000717</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000717</idno>
<idno type="wicri:Area/Pmc/Curation">000717</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000717</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000428</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000428</idno>
<idno type="wicri:Area/Ncbi/Merge">000666</idno>
<idno type="wicri:Area/Ncbi/Curation">000666</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000666</idno>
<idno type="wicri:doubleKey">0022-538X:2017:To J:channel:inactivating:mutations</idno>
<idno type="wicri:Area/Main/Merge">000F26</idno>
<idno type="wicri:Area/Main/Curation">000F24</idno>
<idno type="wicri:Area/Main/Exploration">000F24</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Channel-Inactivating Mutations and Their Revertant Mutants in the Envelope Protein of Infectious Bronchitis Virus</title>
<author><name sortKey="To, Janet" sort="To, Janet" uniqKey="To J" first="Janet" last="To">Janet To</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">School of Biological Sciences, Nanyang Technological University, Singapore, Singapore</nlm:aff>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>School of Biological Sciences, Nanyang Technological University, Singapore</wicri:regionArea>
<wicri:noRegion>Singapore</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Surya, Wahyu" sort="Surya, Wahyu" uniqKey="Surya W" first="Wahyu" last="Surya">Wahyu Surya</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">School of Biological Sciences, Nanyang Technological University, Singapore, Singapore</nlm:aff>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>School of Biological Sciences, Nanyang Technological University, Singapore</wicri:regionArea>
<wicri:noRegion>Singapore</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Fung, To Sing" sort="Fung, To Sing" uniqKey="Fung T" first="To Sing" last="Fung">To Sing Fung</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">School of Biological Sciences, Nanyang Technological University, Singapore, Singapore</nlm:aff>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>School of Biological Sciences, Nanyang Technological University, Singapore</wicri:regionArea>
<wicri:noRegion>Singapore</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Li, Yan" sort="Li, Yan" uniqKey="Li Y" first="Yan" last="Li">Yan Li</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">School of Biological Sciences, Nanyang Technological University, Singapore, Singapore</nlm:aff>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>School of Biological Sciences, Nanyang Technological University, Singapore</wicri:regionArea>
<wicri:noRegion>Singapore</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Verdia Baguena, Carmina" sort="Verdia Baguena, Carmina" uniqKey="Verdia Baguena C" first="Carmina" last="Verdià-Bàguena">Carmina Verdià-Bàguena</name>
<affiliation wicri:level="1"><nlm:aff id="aff2">Department of Physics, Laboratory of Molecular Biophysics. Universitat Jaume I, Castelló, Spain</nlm:aff>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Department of Physics, Laboratory of Molecular Biophysics. Universitat Jaume I, Castelló</wicri:regionArea>
<wicri:noRegion>Castelló</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Queralt Martin, Maria" sort="Queralt Martin, Maria" uniqKey="Queralt Martin M" first="Maria" last="Queralt-Martin">Maria Queralt-Martin</name>
<affiliation wicri:level="1"><nlm:aff id="aff2">Department of Physics, Laboratory of Molecular Biophysics. Universitat Jaume I, Castelló, Spain</nlm:aff>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Department of Physics, Laboratory of Molecular Biophysics. Universitat Jaume I, Castelló</wicri:regionArea>
<wicri:noRegion>Castelló</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Aguilella, Vicente M" sort="Aguilella, Vicente M" uniqKey="Aguilella V" first="Vicente M." last="Aguilella">Vicente M. Aguilella</name>
<affiliation wicri:level="1"><nlm:aff id="aff2">Department of Physics, Laboratory of Molecular Biophysics. Universitat Jaume I, Castelló, Spain</nlm:aff>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Department of Physics, Laboratory of Molecular Biophysics. Universitat Jaume I, Castelló</wicri:regionArea>
<wicri:noRegion>Castelló</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Liu, Ding Xiang" sort="Liu, Ding Xiang" uniqKey="Liu D" first="Ding Xiang" last="Liu">Ding Xiang Liu</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">School of Biological Sciences, Nanyang Technological University, Singapore, Singapore</nlm:aff>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>School of Biological Sciences, Nanyang Technological University, Singapore</wicri:regionArea>
<wicri:noRegion>Singapore</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Torres, Jaume" sort="Torres, Jaume" uniqKey="Torres J" first="Jaume" last="Torres">Jaume Torres</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">School of Biological Sciences, Nanyang Technological University, Singapore, Singapore</nlm:aff>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>School of Biological Sciences, Nanyang Technological University, Singapore</wicri:regionArea>
<wicri:noRegion>Singapore</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">Journal of Virology</title>
<idno type="ISSN">0022-538X</idno>
<idno type="eISSN">1098-5514</idno>
<imprint><date when="2017">2017</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><title>ABSTRACT</title>
<p>It has been shown previously in the severe acute respiratory syndrome coronavirus (SARS-CoV) that two point mutations, N15A and V25F, in the transmembrane domain (TMD) of the envelope (E) protein abolished channel activity and led to <italic>in vivo</italic>
attenuation. Pathogenicity was recovered in mutants that also regained E protein channel activity. In particular, V25F was rapidly compensated by changes at multiple V25F-facing TMD residues located on a neighboring monomer, consistent with a recovery of oligomerization. Here, we show using infected cells that the same mutations, T16A and A26F, in the gamma-CoV infectious bronchitis virus (IBV) lead to, in principle, similar results. However, IBV E A26F did not abolish oligomer formation and was compensated by mutations at N- and C-terminal extramembrane domains (EMDs). The C-terminal EMD mutations clustered along an insertion sequence specific to gamma-CoVs. Nuclear magnetic resonance data are consistent with the presence of only one TMD in IBV E, suggesting that recovery of channel activity and fitness in these IBV E revertant mutants is through an allosteric interaction between EMDs and TMD. The present results are important for the development of IBV live attenuated vaccines when channel-inactivating mutations are introduced in the E protein.</p>
<p><bold>IMPORTANCE</bold>
The ion channel activity of SARS-CoV E protein is a determinant of virulence, and abolishment of channel activity leads to viral attenuation. E deletion may be a strategy for generating live attenuated vaccines but can trigger undesirable compensatory mechanisms through modifications of other viral proteins to regain virulence. Therefore, a more suitable approach may be to introduce small but critical attenuating mutations. For this, the stability of attenuating mutations should be examined to understand the mechanisms of reversion. Here, we show that channel-inactivating mutations of the avian infectious bronchitis virus E protein introduced in a recombinant virus system are deficient in viral release and fitness and that revertant mutations also restored channel activity. Unexpectedly, most of the revertant mutations appeared at extramembrane domains, particularly along an insertion specific for gammacoronaviruses. Our structural data propose a single transmembrane domain in IBV E, suggesting an allosteric interaction between extramembrane and transmembrane domains.</p>
</div>
</front>
</TEI>
<affiliations><list><country><li>Espagne</li>
<li>Singapour</li>
</country>
</list>
<tree><country name="Singapour"><noRegion><name sortKey="To, Janet" sort="To, Janet" uniqKey="To J" first="Janet" last="To">Janet To</name>
</noRegion>
<name sortKey="Fung, To Sing" sort="Fung, To Sing" uniqKey="Fung T" first="To Sing" last="Fung">To Sing Fung</name>
<name sortKey="Li, Yan" sort="Li, Yan" uniqKey="Li Y" first="Yan" last="Li">Yan Li</name>
<name sortKey="Liu, Ding Xiang" sort="Liu, Ding Xiang" uniqKey="Liu D" first="Ding Xiang" last="Liu">Ding Xiang Liu</name>
<name sortKey="Surya, Wahyu" sort="Surya, Wahyu" uniqKey="Surya W" first="Wahyu" last="Surya">Wahyu Surya</name>
<name sortKey="Torres, Jaume" sort="Torres, Jaume" uniqKey="Torres J" first="Jaume" last="Torres">Jaume Torres</name>
</country>
<country name="Espagne"><noRegion><name sortKey="Verdia Baguena, Carmina" sort="Verdia Baguena, Carmina" uniqKey="Verdia Baguena C" first="Carmina" last="Verdià-Bàguena">Carmina Verdià-Bàguena</name>
</noRegion>
<name sortKey="Aguilella, Vicente M" sort="Aguilella, Vicente M" uniqKey="Aguilella V" first="Vicente M." last="Aguilella">Vicente M. Aguilella</name>
<name sortKey="Queralt Martin, Maria" sort="Queralt Martin, Maria" uniqKey="Queralt Martin M" first="Maria" last="Queralt-Martin">Maria Queralt-Martin</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV2/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000F24 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000F24 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Sante |area= CovidV2 |flux= Main |étape= Exploration |type= RBID |clé= PMC:5309962 |texte= Channel-Inactivating Mutations and Their Revertant Mutants in the Envelope Protein of Infectious Bronchitis Virus }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:27974570" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \ | NlmPubMed2Wicri -a CovidV2
This area was generated with Dilib version V0.6.33. |